Establishing efficient siRNA knockdown in mouse embryonic stem cells
β Scribed by Stephen Chen; Andre Choo; Nai-dy Wang; Heng-Phon Too; Steve Kah Weng Oh
- Publisher
- Springer Netherlands
- Year
- 2006
- Tongue
- English
- Weight
- 218 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The mycotoxin citrinin (CTN) is a natural contaminant in foodstuffs and animal feeds, and exerts cytotoxic and genotoxic effects on various mammalian cells. CTN causes cell injury, including apoptosis. However, its precise regulatory mechanisms of action, particularly in stem cells and
## Abstract Recombinaseβmediated cassette exchange (RMCE), when applied to mouse embryonic stem (ES) cells, promises to increase the ease with which genetic alterations can be introduced into targeted genomic loci in the mouse. However, existing selection strategies for identifying ES cells in whic