ESR determinations of membrane permeabil
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F.W. Kleinhans; N.D. Lees; M. Bard; R.A. Haak; R.A. Woods
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Article
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1979
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Elsevier Science
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English
โ 563 KB
Yeast sterol mutants were subjected to ESR analysis in an attempt to elucidate how altered sterol composition correlates with membrane permeability. The technique requires spin labeling the intact yeast cells with a small, water-soluble nitroxide probe (2,2,5,5 tetramethyl-3-pyrrolin-l-oxyl-3-carbox