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ESR determination of membrane order parameter in yeast sterol mutants

โœ Scribed by Norman D Lees; Martin Bard; Marvin D Kemple; Richard A Haak; Frederick W Kleinhans


Book ID
115724317
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
421 KB
Volume
553
Category
Article
ISSN
0005-2736

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ESR determinations of membrane permeabil
โœ F.W. Kleinhans; N.D. Lees; M. Bard; R.A. Haak; R.A. Woods ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 563 KB

Yeast sterol mutants were subjected to ESR analysis in an attempt to elucidate how altered sterol composition correlates with membrane permeability. The technique requires spin labeling the intact yeast cells with a small, water-soluble nitroxide probe (2,2,5,5 tetramethyl-3-pyrrolin-l-oxyl-3-carbox