Escherichia coli: Reversion from streptomycin dependence, a mutation in a specific 30 s ribosomal protein
β Scribed by Apirion, D.; Schlessinger, D.; Phillips, S.; Sypherd, P.
- Book ID
- 123413316
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 779 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0022-2836
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The conditionally lethal mutation, 2861 mis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and was found to cotransduce at 97% with rpsE (S5). The 2861 mis mutation leads to thermosensitivity and impaired assembly in vivo of 30S ribosomal
Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of Escherichia coli K12. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon. One of