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Escherichia coli resistance to β-lactam antibiotics through a decrease in the affinity of a target for lethality

✍ Scribed by SPRATT, BRIAN G.


Book ID
109710441
Publisher
Nature Publishing Group
Year
1978
Tongue
English
Weight
385 KB
Volume
274
Category
Article
ISSN
0028-0836

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The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G----T, leading to amino acid