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Escherichia coli parA is an allele of the gyrB gene

✍ Scribed by Kato, Jun-ichi ;Nishimura, Yukinobu ;Suzuki, Hideho

Publisher: Springer
Year: 1989
Tongue: English
Weight: 556 KB
Volume: 217
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330959

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✦ Synopsis

A thermosensitive (ts) parA mutant, MFT110, of Escherichia coli carried at least two ts mutations. The major ts defect, resulting from a mutation mapped originally at 95 min and complemented by pLC8-47, was most probably due to psd. A plasmid carrying the 1.6 kb BamHI-PvuII fragment recloned from pLC8-47 complemented the major ts mutation in MFT110 and psd(ts) in two mutants, but did not correct the Par phenotype of MFT110. The second ts mutation was salt-repairable and mapped at 83 min close to recF and tnaA. This mutation was linked with the Par phenotype as shown unambiguously by 4',6-diamidino-2-phenylindole stained nucleoids in parA mutant cells with the W3110 genetic background. Both salt-repairable ts and Par traits were corrected concomitantly by a plasmid carrying the chromosomal region solely for the gyrB gene. This strongly suggests that parA is an allele of gyrB.

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