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Escherichia coli mutY Gene Product is Required for Specific A-G→ C· G Mismatch Correction

✍ Scribed by K. G. Au, M. Cabrera, J. H. Miller and Paul Modrich

Book ID
123636355
Publisher
National Academy of Sciences
Year
1988
Tongue
English
Weight
760 KB
Volume
85
Category
Article
ISSN
0027-8424

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G:C → T:A and G:C → C:G transversions ar
G:C → T:A and G:C → C:G transversions are the predominant spontaneous mutations in the Escherichia coli supF gene: an improved lacZ(am) E. coli host designed for assaying pZ189 supF mutational specificity
✍ Akasaka, Susumu ;Takimoto, Koichi ;Yamamoto, Kazuo 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 682 KB

Escherichia coli K12 strain KS40 and plasmid pKY241 were designed for easy screening of supF mutations in plasmid pZ189. KS40 is a nalidixic acid-resistant (gyrA) derivative of MBM7070 (lacZ(am)CA7020). Using in vitro mutagenesis, an amber mutation was introduced into the cloned gyrA structural gene