Escherichia coli hydrogenase activity and H2 production under glycerol fermentation at a low pH

✍ Scribed by Karen Trchounian; Viviana Sanchez-Torres; Thomas K. Wood; Armen Trchounian

• Book ID: 103833000
• Publisher: Elsevier Science
• Year: 2011
• Tongue: English
• Weight: 581 KB
• Volume: 36
• Category: Article
• ISSN: 0360-3199
• DOI: 10.1016/j.ijhydene.2010.12.128

✦ Synopsis

Hydrogenase (Hyd) activity and H 2 production by Escherichia coli were studied at a low pH.

H 2 production at pH 5.5 under glycerol fermentation was shown to be w1.5-fold higher than that at pH 6.5 or above but less than that under glucose fermentation. It was inhibited by N,N 0 -dicyclohexylcarbodiimide: H 2 production inhibition was increased with decreasing pH and almost maximal inhibition was observed at pH 5.5. The data on H 2 production by single and double mutants with defects in different Hyd-enzymes and in fhlA gene suggest that under glycerol fermentation at a low pH, Hyd-1, Hyd-2 and Hyd-4 were operating in a reversed, non-H 2 producing mode. Moreover, a role of fhlA gene in Hyd-3 and Hyd-4 activity in H 2 production is proposed under glucose fermentation at a low pH.