ES cells do not activate p53-dependent stress responses and undergo p53-independent apoptosis in response to DNA damage
โ Scribed by Mirit I. Aladjem; Benjamin T. Spike; Luo Wei Rodewald; Thomas J. Hope; Martina Klemm; Rudolf Jaenisch; Geoffrey M. Wahl
- Book ID
- 114320991
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 167 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0960-9822
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๐ SIMILAR VOLUMES
experiments shown in Fig. , b were exactly as described 5 , except that the binding and washing buffer for the GST -LHP1 results shown in Fig. consisted of 50 mM sodium phosphate and 25 mM NaCl at pH 6.0. For Fig. , bound proteins were separated by 4 -20% gradient SDS -PAGE, blotted, and detected wi
Exposure of mammalian cells to genotoxic agents evokes a complex cellular response. An ordered series of molecular events is necessary to sense DNA damage, transduce the signal, and ultimately delay the cell cycle or trigger apoptosis. Recently, we have shown that BTG2/TIS21 gene expression was indu