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Erythropoietic progenitors capable of colony formation in culture: State of differentiation

✍ Scribed by C. J. Gregory; E. A. McCulloch; J. K. Till

Book ID
102882354
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
632 KB
Volume
81
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The differentiated state of mouse erythropoietic progenitor cells (CFU‐E), detected by their ability to form erythropoietin‐dependent colonies in vitro, has been investigated. Transfusion‐induced plethora was found to reduce the population size of CFU‐E in both spleen and femoral marrow, which indicates that a significant number of CFU‐E arise by differentiation processes that are themselves erythropoietin‐dependent. Individual spleen colonies were found to be heterogeneous in their content of CFU‐E, and the numbers of CFU‐E per colony were not correlated either positively or negatively with the numbers of granulocyte‐macrophage progenitors (CFU‐C) present in the same colonies. The absence of a negative correlation between CFU‐E and CFU‐C indicates that the erythropoietic and granulopoietic pathways of differentiation are not mutually exclusive within individual spleen colonies. The numbers of CFU‐E per spleen colony were also found to vary independently of the numbers of pluripotent stem cells (CFU‐S) per colony; in contrast, as found previously, the numbers of CFU‐C and CFU‐S per colony were positively correlated. These results indicate that more randomizing events separate CFU‐E from CFU‐S than separate CFU‐C from CFU‐S, and are consistent with the view that CFU‐E occupy a position on the erythropoietic pathway of differentiation that is more remote from the pluripotent stem cells than is the corresponding position of CFU‐C on the granulopoietic pathway.

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