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ERK2 Signalling from Internalised Epidermal Growth Factor Receptor in Broken A431 Cells

โœ Scribed by Luzheng Xue; John Lucocq

Book ID
114284142
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
417 KB
Volume
10
Category
Article
ISSN
0898-6568

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Rapid isolation of plasmalemma from cult
Rapid isolation of plasmalemma from cultured A431 cells: Characterization of epidermal growth factor receptors
โœ Peter H. Lin; Richard H. Selinfreund; Eric Wakshull; Walker Wharton ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 507 KB

A rapid method for the purification of plasma membrane from a relatively small number of A431 cells is described. The method is a simple, two-step differential centrifugation in the presence of Ca2+ that requires a total centrifugation time of 7 min. The membrane preparations contained a high level