Ergosterol as an indicator of mould growth on wood in relation to culture age, humidity stress and nutrient level
✍ Scribed by J. Bjurman
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 637 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0964-8305
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✦ Synopsis
Changes in ergosterol content in cultures of Penicillium brevicompactum and Aspergillus versicolor on wood with time, changes" in humidity or addition of glucose solutions to wood were studied with HPLC. Lowering Of the humidi O, level caused a very large decline in ergosterol content (~]'cultures 0/" P. brevicompactum on wood over a 10 day period, although small amounts remained qfier this" time. After an initial increase, up to an inoculation time of 45 days, reductions were also observed in control samples maintained at 100% RH, but these were smaller. The amount o/ ergosterol decreased to very low levels in wood impregnated with low levels (~['glucose during a 93 day incubation period. Ergosterol concentration in hyphae produced in surJitce liquid cultures was shown to be higher in myeelia growing on media enriched with nitrogen or with more available nutrients. The concentration ~[" ergosterol in the mycelia o/" P. brevicompactum in surface liquid cultures varied by a factor of 5 from 2 to 10 mg g ~. The results clearly show that ergosterol present in solid materials is main O' related to active biomass. With certain prerequisites, ergosterol determinations could also be used Jbr total fungal biomass estimations on wood.