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Equilibrium protein folding-unfolding process involving multiple intermediates

✍ Scribed by Hui-Chih Hung; Yu-Hou Chen; Guang-Yaw Liu; Hwei-Jen Lee; Gu-Gang Chang

Publisher
Springer
Year
2003
Tongue
English
Weight
302 KB
Volume
65
Category
Article
ISSN
1522-9602

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✦ Synopsis

Mathematical models for the protein folding-unfolding process involving multiple intermediates have been derived. Computer fitting of the experimental data to this model generates various thermodynamic parameters for the folding-unfolding process. In this way, the complex folding-unfolding process of the multi-domain proteins can be analysed in a quantitative way. The application of the folding-unfolding model involving seven stages in human placental alkaline phosphatase is described.

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