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Equilibrium Gel Filtration Chromatography for the Measurement of Protein-Ligand Binding in the Undergraduate Biochemistry Laboratory

✍ Scribed by Craig, Douglas B.

Book ID
126930795
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
82 KB
Volume
82
Category
Article
ISSN
0021-9584

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## Abstract Enzymes with ribonucleolytic activity play a pivotal role in gene expression and cellular homeostasis by altering the levels of cellular RNA. Ribonuclease A has been the most well studied of such enzymes whose histidine residues (His^12^ and His^119^) play a crucial role in the catalyti