Equilibrium adsorption of poly(His)-tagged proteins on immobilized metal affinity chromatographic adsorbents
✍ Scribed by Yi-Hsing Yang; Tusng-Ta Wu; Shing-Yi Suen; Sung-Chyr Lin
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 404 KB
- Volume
- 54
- Category
- Article
- ISSN
- 1369-703X
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract The covalent and non‐covalent immobilization of growth factors such as recombinant human bone morphogenetic protein 2 (rhBMP‐2) on metals and bone replacement materials in bioactive form is a recent development. Up to now the immobilization technology usually involved the chemical modif
The facilitated downstream processing of an intracellular, polyhistidine-tagged protein, glutathione Stransferase [GST-(His) 6 ], direct from unclarified E. coli homogenates using expanded beds of STREAMLINE chelating, has been investigated. A series of pilot experiments were used to develop prepara
## Abstract Epoxy supports covalently immobilize proteins following a two‐step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i