𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Epstein-Barr virus strain- and group-specific antigenic determinants detected by monoclonal antibodies

✍ Scribed by Nikolaus Mueller-Lantzsch; Brigitte Georg-Fries; Hermann Herbst; Harald Zur Hausen; Dietmar G. Braun

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
694 KB
Volume
28
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In order to differentiate Epstein‐Barr virus (EBV) strains by serological markers we prepared hybridomas producing monoclonal antibodies against polypeptides of the QIMR‐WIL EBV strain. These monoclonal antibodies were screened by indirect immunofluorescence techniques. Immunoprecipitation, using EBV‐positive monoclonal antibodies and the protein A method, of ^125^I‐labelled polypeptides from purified QIMR‐WIL EBV particles, revealed that a series of monoclonal antibodies against the four main surface and envelope polypeptides precipitating p^340^, p340/p240, p140, and p80 were obtained. When ^125^I‐labelled polypeptides from purified P3HR‐I and B95‐8 EBV particles were immunoprecipitated, it could be demonstrated that several anti‐p340 (QIMR‐WIL) antibodies recognized strainspecific antigenic determinants, while anti‐p340/p240 (QIMR‐WIL) as well as anti‐p140 (QIMR‐WIL) antibody clones reacted with antigenic sites which are in common wither among B95‐8 or in addition to P3HR‐I polypeptides. Thus, monoclonal antibodies now provide a serological basis for EBV typing.

📜 SIMILAR VOLUMES

Detection of epstein-barr virus early an
Detection of epstein-barr virus early antigen-d and its antibodies by passive hemagglutination
✍ Clifton A. Ogburn; Barbara Ann Zajac 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 815 KB

## Abstract These data describe the use of a passive hemagglutination test (PHAT) for the detection of antibodies to the Epstein‐Barr virus (EBV) associated antigen, early antigen‐D (EA‐D). EA‐D was obtained from EBV‐superinfected RPMI 64–10 cell preparations which showed by immunofluorescent stain

Monoclonal antibodies to epstein-barr vi
Monoclonal antibodies to epstein-barr virus-induced, transformation-associated cell surface antigens: Binding patterns and effect upon virus-specific t-cell cytotoxicity
✍ M. Rowe; A. B. Rickinson; M. A. Epstein; J. E. K. Hildreth 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 French ⚖ 1001 KB

## Abstract Spleen cells from mice immunized with Epstein‐Barr virus‐transformed lymphoblastoid cells (EB‐LCL) were used to generate monoclonal antibodies to cell surface antigens associated with the EB virus‐transformed state. Radioimmune and immunofluorescence binding assays identified two antibo

Antibodies to Epstein-Barr virus-specifi
Antibodies to Epstein-Barr virus-specific DNase in patients with nasopharyngeal carcinoma and control groups
✍ Jen-Yang Chen; Chien-Jen Chen; Mei-Ying Liu; Show-Mei Cho; Mow-Ming Hsu; Tsong-C 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 660 KB

Serum samples from 154 patients with nasopharyngeal carcinoma (NPC), 374 with other cancers, 1,000 normal controls from Government Employees' Clinic Center (GECC), and 3,642 individuals of various ethnic-dialect groups living in high-risk areas for NPC were collected and the concentration of antibod

Epstein-Barr virus specific salivary ant
Epstein-Barr virus specific salivary antibodies as related to stress caused by examinations
✍ O. Sarid; O. Anson; A. Yaari; M. Margalith 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB

## Abstract Epstein‐Barr virus (EBV) is prevalent in 90% of the population. After primary infection it remains in a latent state and the majority of the virus carriers are asymptomatic during their life. Among the immunocompromized patients such as organ and bone marrow transplant recipients, indiv