Epstein-Barr Virus Protocols

✍ Scribed by Paul J. Farrell (auth.), Joanna B. Wilson, Gerhard H. W. May (eds.)

• Publisher: Humana Press
• Year: 2001
• Tongue: English
• Leaves: 453
• Series: Methods in Molecular Biology 174
• Edition: 1
• Category: Library

✦ Synopsis

The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels.
Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.

✦ Table of Contents

Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Front Matter....Pages 3-12
Front Matter....Pages 13-22
Front Matter....Pages 23-35
Front Matter....Pages 37-43
Front Matter....Pages 45-66
Front Matter....Pages 67-76
Front Matter....Pages 77-77
Front Matter....Pages 79-91
Back Matter....Pages 93-102
....Pages 103-110

✦ Subjects

Immunology