Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non-Hodgkin's lymphoma of human immunodeficiency virus-negative Japanese

✍ Scribed by Ohshima, Koichi; Suzumiya, Junji; Tasiro, Kotaro; Mukai, Yasuo; Tanaka, Tosihiro; Kato, Akiko; Kikuchi, Masahiro

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 930 KB
• Volume: 52
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/(sici)1096-8652(199605)52:1<21::aid-ajh4>3.0.co;2-8

✦ Synopsis

Sixty cases of B-cell nodal nonHodgkin's malignant lymphoma (B-ML), and 46 cases of T-cell nodal lymphoma (T-ML) were surveyed for Epstein-Barr virus (EBV) genomes, RNA, and associated proteins. We used a Southern blot analysis, polymerase chain reaction (PCR), and EBV-encoded small RNA-1 (EBER-1) in situ hybridization to investigate the presence of EBV. We performed an imrnunohistochernical study on EBV-related oncoproteins, such as EBV-determined nuclear antigen9 (EBNA-2), latent membrane protein (LMP), and viral interleukin-10 (vlL-10). In addition, we also analyzed the terminal repetitive sequence of EBV (EBV-TR) to investigate the EBV-infected cell clonality. Non-Hodgkin's lymphomas were grouped into three types by number of EBV-infected cells: I) almost all lymphoma cells showed an EBV presence; II) some scattered lymphoma cells showed an EBV presence; and 111) only a few cells showed such a presence, which was probably due to a latent EBV infection. In 25 of 60 B-MLs, EBV-infected cells were found; 7 were type I, 1 was type II, and 17 were type 111. In 27 of 46 T-MLs, EBV-infected cells were found; no cases were type I, 5 cases were type II, and 22 cases were type 111. Seven B-MLs and 3 T cell lymphomas showed clonal TR bands. Expression of EBNA-2 was found in only three B-MLs, whereas LMP was seen in four B-MLs and six T-MLs. All EBNA-ZLMPpositive cases showed an EBV presence. In B-MLs, expression of EBNA-2 and LMP was detected in almost all lymphoma cells: in T-MLs, however, LMP was found in only a small portion of the lymphoma cells. Expression of IL-10 was closely associated with LMP. In summary, it was thus speculated that EBV infection was associated with the various states of lymphomagenesis.