Epstein-Barr-virus-infected human T-cell line with a unique pattern of viral-gene expression

✍ Scribed by Hiroyuki Nakamura; Dai Iwakiri; Yasushi Ono; Shigeyoshi Fujiwara

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: French
• Weight: 382 KB
• Volume: 76
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19980518)76:4<587::aid-ijc23>3.0.co;2-3

✦ Synopsis

Epstein-Barr-virus(EBV)-gene expression was analyzed in clonal sub-lines of the human T-cell line MT-2 that are persistently infected by the virus with a positive selection marker. Immunoblot analyses showed the expression of EBV proteins associated with the latent viral cycle, including the EBV nuclear antigen 1 (EBNA1) and the latent membrane protein 1 (LMP1), whereas EBNA2 was not detected. RT-PCR further demonstrated the expression of LMP2A, EBVencoded small RNA 1 (EBER1), and complementary-strand transcripts from the BamHI-A fragment. EBNA2 mRNA was detected at a very low level. In addition to these latent viral functions, the BZLF1 protein, a transcription factor that activates a cascade of replicative-gene expression in EBVinfected B cells, was detected in these MT-2 clones by immunoblot analysis and immuno-enzymatic staining. Moreover, mRNA from other genes of the early virus-replicative cycle, such as BRLF1, BSMLF1, and BALF2, as well as BZLF1 was detected by Northern-blot analysis. Since sub-lines of the human B-cell lines BJAB and Louckes infected with similar recombinant EBV did not express these early genes, these data suggest that MT-2 cells are relatively permissive for expression of early EBV-replicative functions.