𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Epstein-Barr virus-associated complement-fixing and nuclear antigens in Burkitt lymphoma biopsies

✍ Scribed by Beverley M. Reedman; G. Klein; J. H. Pope; Marilyn K. Walters; J. Hilgers; S. Singh; B. Johansson

Book ID
102867020
Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
626 KB
Volume
13
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Cells from Burkitt lymphoma (BL) biopsies were examined for Epstein‐Barr virus (EBV)‐associated antigens by complement fixation (CF) tests and by the anti‐complement immunofluorescence (ACIF) test. In CF tests, anticomplementary factors made it difficult to test all the biopsies available. However, biopsies from 19 patients were effectively tested and 12 of these (including two from one patient) contained antigen reacting with a battery of human sera with antibody to EBV but not with sera lacking such antibody. Technical difficulties prevented further characterization of the EBV‐related antigens in the biopsies. Application of the ACIF test to BL revealed the presence of EBV‐related nuclear antigen in biopsies from 11 of 13 patients. Absorption studies indicated that the nuclear antigens of the biopsies were closely related antigenically to the EBV‐determined nuclear antigen (EBNA) previously described in lymphoblastoid cell lines. It is concluded that cells of BL biopsies may contain EBNA in addition to the EBV‐related membrane antigen previously described. The results provide further evidence that BL cells from African patients resemble non‐producer lymphoblastoid cell lines in containing EBNA and therefore appear to be transformed by EBV.

📜 SIMILAR VOLUMES

Association between Epstein-Barr virus a
Association between Epstein-Barr virus and Burkitt's lymphoma in Taiwan
✍ Tsu-Yi Chao; Tow-Yeuan Wang; Wei-Hua Lee 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 550 KB

## Background: The association between epstein-barr virus (ebv) and burkitt's lymphoma (bl) in taiwan is not clear. in this study, the authors attempted to determine the frequency of the occurrence of ebv infection in patients with bl in taiwan. ## Methods: A retrospective study was performed usi

Sensitive method to detect non-complemen
Sensitive method to detect non-complement-fixing antibodies to Epstein-Barr virus nuclear antigen
✍ H. Schmitz; D. Kampa; W. Heidenreich 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 327 KB 👁 1 views

## Abstract The anti‐complement immunofluorescence (ACIF) test was modified to detect non‐complement‐fixing antibodies to Epstein‐Barr (EB) virus nuclear antigen (EBNA). These EBNA antibodies belong to the immunoglobulin classes IgA and IgG. In our method anti‐human γ‐globulin (AHG) was bound to th

Relationship between Epstein-Barr virus
Relationship between Epstein-Barr virus (EBV) DNA and the EBV-determined nuclear antigen (EBNA) in Burkitt lymphoma biopsies and other lymphoproliferative malignancies
✍ Tomas Lindahl; George Klein; Beverley M. Reedman; Bo Johansson; Surjit Singh 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 649 KB

## Abstract Twenty‐six of 27 African Burkitt lymphomas with histologically confirmed diagnosis contained relatively large amounts of EBV DNA (10–101 viral genomes per cell), as determined by nucleic acid hybridization. Twenty‐five of the 26 EBV DNA‐positive lymphomas contained the EBV‐determined nu

Demonstration of epstein-barr virus-asso
Demonstration of epstein-barr virus-associated nuclear antigen in nasopharyngeal carcinoma cells from fresh biopsies
✍ D. P. Huang; J. H. C. Ho; W. Henle; G. Henle 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 862 KB

## Abstract Fresh nasopharyngeal carcinoma (NPC) biopsies were treated in several ways to yield satisfactory cell preparations for detection of Epstein‐Barr virus (EBV)‐associated nuclear antigen (EBNA) by anti‐complement immunofluorescence tests. If the cells were well dispersed (trypsinization or