EPR imaging in biological applications: Towards microtomography

✍ Scribed by A. Sotgiu; G. Placidi; G. Gualtieri; C. Tatone; C. Campanella

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 511 KB
• Volume: 33
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1260331324

✦ Synopsis

Abstract

EPR imaging and microtomography promises to be a powerful tool for the EPR spectroscopist, but the expansion of this technique was hindered by the lack of commercial apparatus. A simple modification of an existing commercial system that can reach a resolution of the order of 75 μm with a gradient of 150 mT m^−1^ is described. This resolution can be increased by using larger field gradients. In many biological applications this resolution is significant. As an example, the distribution of a paramagnetic probe in an amphibious egg cell is presented that permits one to distinguish the nucleus of the cell. A summary of the mathematical techniques which enable one to account for the presence of a hyperfine structure in most EPR paramagnetic probes and to increase resolution is also presented.