Epitopes immunologically related to glycophorin a on human malignant and non-malignant cells in culture
✍ Scribed by A. L. Barsoum; M. S. Czuczman; V. P. Bhavanandan; E. A. Davidson
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- French
- Weight
- 828 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Nine monoclonal antibodies specific to different antigenic determinants on the cyanogen bromide fragments CNBr3 (I -8) and CNBrl (9-8 I) of glycophorin A, the major sialoglycoprotein of human red blood cells, were used for the detection of similar or cross-reactive epitope(s) on nucleated somatic cells. A panel of human malignant and non-malignant cells consisting of erythroleukemia. melanoma and carcinomas of breast, cervix, larynx, nasopharynx and colon, as well as human fibroblasts and mammary cells, were tested using a sensitive enzyme-linked immunoway. The presence of glycophorin-like epitopes were demonstrated, in varying concentrations, on all cells tested. These epitropes were found to be localized on the cell membranes by indirect immunofluorescence.