Epitopes for human monoclonal antibodies and serotyping antisera against the O-specific polysaccharide of Pseudomonas aeruginosa O11
✍ Scribed by Shin-ichi Yokota; Hiroshi Noguchi
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 616 KB
- Volume
- 261
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Epitopes for Pseudomonas aeruginosa 011-specific human monoclonal antibodies (mAbs) and 011 serotyping antisera have been characterized . These mAbs recognized the O-polysaccharide portion of the lipopolysaccharide . The structure of the 0-polysaccharide of Oll has been reported to be comprised of trisaccharide repeating-units as follows : --* 3)-a-i.-ucpNAc-(1 -* 3)-fi-n-ucpNAc-(1 -, 2)-$-n-Glcp-(l --+ . ( ucpNAc, 2-acetamido-2,6-dideoxygalactopyranoside .) Data from inhibition studies of binding in enzyme-linked immunosorbent assays and cell-agglutination assays, using monosaccharides and periodateoxidized 0-polysaccharide showed that the glucose residue, especially the C-3-C-6 segment and the S-anomeric configuration, in the polysaccharide is essential for the epitopes of all anti-011 mAbs ; however, the detailed epitope specificities were different from one another . urthermore, epitopes for serotyping antisera of 011 seemed to be similar to those for the human mAbs.