Epithelial Cell Culture

✍ Scribed by Mario Baratta

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 216
• Series: Methods in Molecular Biology 1817
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.

✦ Table of Contents

Front Matter ....Pages i-xiii
Normal Human Thyrocytes in Culture (Sarah J. Morgan, Susanne Neumann, Marvin C. Gershengorn)....Pages 1-7
Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia (James D. Lillich, Peying Fong)....Pages 9-18
Reassembly of Functional Human Stem/Progenitor Cells in 3D Culture (Danielle Wu, Patricia Chapela, Mary C. Farach-Carson)....Pages 19-32
Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro (Dahai Zheng, Jianzhu Chen)....Pages 33-40
Differentiation of Gastrointestinal Cell Lines by Culture in Semi-wet Interface (Macarena P. Quintana-Hayashi, Sara K. Lindén)....Pages 41-46
Three-Dimensional Cell Culture Model Utilization in Renal Carcinoma Cancer Stem Cell Research (Kamila Maliszewska-Olejniczak, Klaudia K. Brodaczewska, Zofia F. Bielecka, Anna M. Czarnecka)....Pages 47-66
Amniotic Epithelial Cell Culture (Angelo Canciello, Luana Greco, Valentina Russo, Barbara Barboni)....Pages 67-78
Bovine Granulosa Cell Culture (Bushra T. Mohammed, F. Xavier Donadeu)....Pages 79-87
Bioencapsulation of Oocytes and Granulosa Cells (Massimo Faustini, Giulio Curone, Maria L. Torre, Daniele Vigo)....Pages 89-93
Ovine Granulosa Cells Isolation and Culture to Improve Oocyte Quality (Giovanni Giuseppe Leoni, Salvatore Naitana)....Pages 95-106
3D Model Replicating the Intestinal Function to Evaluate Drug Permeability (Inês Pereira, Anna Lechanteur, Bruno Sarmento)....Pages 107-113
Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture (Jinhua Qin, Xuetao Pei)....Pages 115-121
Long-Term Culture of Intestinal Organoids (Seung Bum Lee, Sung-Hoon Han, Sunhoo Park)....Pages 123-135
Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells (Eugenio Martignani, Paolo Accornero, Silvia Miretti, Mario Baratta)....Pages 137-144
Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids (Emma Teal, Nina Bertaux-Skeirik, Jayati Chakrabarti, Loryn Holokai, Yana Zavros)....Pages 145-155
Mouse-Derived Gastric Organoid and Immune Cell Co-culture for the Study of the Tumor Microenvironment (Jayati Chakrabarti, Loryn Holokai, LiJyun Syu, Nina Steele, Julie Chang, Andrzej Dlugosz et al.)....Pages 157-168
Murine and Human Mammary Cancer Cell Lines: Functional Tests (Paolo Accornero, Eugenio Martignani, Silvia Miretti, Mario Baratta)....Pages 169-183
In Vitro Porcine Colon Culture (Matheus O. Costa, Janet E. Hill, Michael K. Dame, John C. S. Harding)....Pages 185-195
Primary Cultures of Olfactory Neurons from the Avian Olfactory Epithelium (George Gomez)....Pages 197-207
Back Matter ....Pages 209-214

✦ Subjects

Life Sciences; Cell Biology