Epigenetic Reprogramming During Mouse Embryogenesis: Methods and Protocols

✍ Scribed by Katia Ancelin, Maud Borensztein

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 320
• Series: Methods in Molecular Biology 2214
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming.

✦ Table of Contents

Front Matter ....Pages i-xi
Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency (Katia Boutourlinsky, Nicolas Allègre, Claire Chazaud)....Pages 1-10
Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos (Sandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, Michel Cohen-Tannoudji)....Pages 11-30
In Vitro Culture of Mouse Blastocysts to the Egg Cylinder Stage via Mural Trophectoderm Excision (Hatice O. Ozguldez, Ivan Bedzhov)....Pages 31-40
Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces (Sophie M. Morgani, Anna-Katerina Hadjantonakis)....Pages 41-58
Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines (Malgorzata Borkowska, Harry G. Leitch)....Pages 59-73
Generation of Primordial Germ Cell-like Cells on Small and Large Scales (Wolfram H. Gruhn, Ufuk Günesdogan)....Pages 75-89
Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions (Valentina Carlini, Kristjan H. Gretarsson, Jamie A. Hackett)....Pages 91-108
Somatic Reprograming by Nuclear Transfer (Vincent Brochard, Nathalie Beaujean)....Pages 109-123
Targeted Transgenic Mice Using CRISPR/Cas9 Technology (Fatima El Marjou, Colin Jouhanneau, Denis Krndija)....Pages 125-141
Whole-Mount Immunofluorescence Staining of Early Mouse Embryos (Frederick C. K. Wong)....Pages 143-155
Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescence In Situ Hybridization (Maud Borensztein)....Pages 157-173
Mapping of Chromosome Territories by 3D-Chromosome Painting During Early Mouse Development (Katia Ancelin, Yusuke Miyanari, Olivier Leroy, Maria-Elena Torres-Padilla, Edith Heard)....Pages 175-187
Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-Seq (Raquel Pérez-Palacios, Patricia Fauque, Aurélie Teissandier, Déborah Bourc’his)....Pages 189-205
Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals (Ambre Bender, Hala Al Adhami, Thomas Dahlet, Michael Weber)....Pages 207-220
Profiling DNA Methylation Genome-Wide in Single Cells (António Galvão, Gavin Kelsey)....Pages 221-240
Tracking Histone Modifications in Embryos and Low-Input Samples Using Ultrasensitive STAR ChIP-Seq (Bingjie Zhang, Xu Peng, Feng Xu, Wei Xie)....Pages 241-252
Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN (Srinivasa Abishek Prakash, Joan Barau)....Pages 253-264
DamID to Map Genome-Protein Interactions in Preimplantation Mouse Embryos (Mrinmoy Pal, Jop Kind, Maria-Elena Torres-Padilla)....Pages 265-282
Understanding Chromosome Structure During Early Mouse Development by a Single-Cell Hi-C Analysis (Noémie Ranisavljevic, Maud Borensztein, Katia Ancelin)....Pages 283-293
Bioinformatic Analysis of Single-Cell Hi-C Data from Early Mouse Embryo (Samuel Collombet, Yuvia A. Pérez-Rico, Katia Ancelin, Nicolas Servant, Edith Heard)....Pages 295-316
Back Matter ....Pages 317-318

✦ Subjects

Life Sciences; Developmental Biology; Genetics and Genomics; Biological Techniques