Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity

✍ Scribed by Yanning Ding; William J. Brackenbury; Pinar U. Onganer; Ximena Montano; Louise M. Porter; Lucy F. Bates; Mustafa B.A. Djamgoz

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 131 KB
• Volume: 215
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.21289

✦ Synopsis

Abstract

The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage‐gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose‐dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co‐application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF‐induced enhancement of migration occurred via VGSC activity. J. Cell. Physiol. 215: 77–81, 2008. © 2007 Wiley‐Liss, Inc.