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Epidermal G1-chalone and transforming growth factor-β are two different endogenous inhibitors of epidermal cell proliferation

✍ Scribed by K. Hartmut Richter; Ruben Schnapke; Matthias Clauss; Gerhard Fürstenberger; Detlef Hinz; Friedrich Marks

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
912 KB
Volume
142
Category
Article
ISSN
0021-9541

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✦ Synopsis

Republic of Germany

Epidermal G,-chalone and transforming growth factor-@ (TGFP), two endogenous inhibitors of epidermal cell proliferation, were compared with regard to several effects on epidermis in vivo and in vitro. Both factors inhibited DNA labeling in a rat tongue epithelial cell line, with similar kinetics and half-maximal effects at approximately 1 pgiml (enriched chalone) and 1 ngiml (TGFP). For primary neonatal mouse keratinocytes, TGFP was found to be a rather strong inhibitor of cell proliferation, whereas chalone showed only a weak effect on cells grown in medium containing 1.2 m M Ca'.' and no effect at all in the presence of 0.06 m M Ca2 + . Vice versa, upon i.p. injection, only chalone was able to inhibit mouse epidermal DNA synthesis in vivo, whereas TGFP had no effect at all. A moderate increase of transglutaminase activity in neonatal primary mouse keratinocytes was induced by both factors at concentrations of about 300 pg TGFP/ml and 10 pg chalone fractioniml. Chalone did not compete with [lL51]TGFP for specific binding sites on primary murine keratinocytes. A polyclonal "chalone antiserum" did not interact with TGFP, and a neutralizing TGFP antibody that inhibited the effect of TGFP on cell proliferation could not block the inhibitory effect of chalone on RTE2 cells. In contrast to TGFP, epidermal G,-chalone did not induce proliferation of NIH-3T3 cells. These results indicate that epidermal G,-chalone and TGFP are two different inhibitors of epidermal cell proliferation.

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