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EP24.15 is associated with lipid rafts

✍ Scribed by Nathaniel A. Jeske; Marc J. Glucksman; James L. Roberts

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
158 KB
Volume
74
Category
Article
ISSN
0360-4012

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✦ Synopsis

Abstract

Metalloendopeptidase EC 3.4.24.15 (EP24.15, thimet oligopeptidase) is a neuropeptide‐metabolizing peptidase expressed throughout the body, but primarily in the brain, gonads, and pituitary. For EP24.15 to have its greatest effect upon peptides in the periphery, it must be targeted and released into the extracellular space. Western blot analysis of fractions taken from discontinuous sucrose density gradients carried out on crude plasma membrane fractions from AtT‐20 cells reveals colocalization of EP24.15 and flotillin‐1, a known lipid raft marker. Further analysis revealed that an intracellular membrane marker and non‐lipid raft, plasma membrane marker, failed to colocalize, supporting EP24.15/lipid raft association. Furthermore, EP24.15 immunoreactivity in lipid raft fractions generated from cells treated with methyl β‐cyclodextrin (MβCD) was greatly reduced. Finally, treatment with MβCD resulted in the accumulation of EP24.15 in the media of drug‐treated cells over vehicle‐treated cells, suggesting that a large percentage of EP24.15 associating with lipid rafts resides on the extracellular surface of the plasma membrane. With this exofacial localization, EP24.15 could have ample access to neuropeptides not only in the immediate microenvironment, but the ability to degrade or modify peptides bound for receptor interaction. © 2003 Wiley‐Liss, Inc.

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