Enzymic synthesis of oligosaccharides on a polymer support. Light-sensitive, substituted polyacrylamide beads

✍ Scribed by Uri Zehavi; Shoshana Sadeh; Mina Herchman

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 738 KB
• Volume: 124
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(83)88352-9

✦ Synopsis

Two light-sensitive, gluco and cellobio polymers, were synthesized by formation of an amide bond between 4-carboxy-2-nitrobenzyl fi-D-glucopyranoside or 4-carboxy-2-nitrobenzyl 4-O-/?-D-glucopyranosyl-P-D-glucopyranoside, respectively, and aminoethyl-substituted, polyacrylamide-gel beads. Subsequently, the polymers were used as acceptors of glycosyltransferase (D-galactosyltransferase, EC 2.4.1.22) and transglycosylase (lysozyme, EC 3.4.1.17) activity. As 2-nitrobenzyl derivatives, the polymers did undergo intramolecular oxidation-reduction upon irradiation (>32Cl nm), followed by the release of the free, reducing oligosaccharides.