Enzymes in cancer. II. The isocitric dehydrogenase of the cerebrospinal fluid in various cancerous and noncancerous conditions

✍ Scribed by Marc van Rymenant; Jacques Robert

• Publisher: John Wiley and Sons
• Year: 1960
• Tongue: English
• Weight: 352 KB
• Volume: 13
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(196009/10)13:5<878::aid-cncr2820130504>3.0.co;2-4

✦ Synopsis

SOCITRIC dehydrogenase is the enzyme that I catalyzes the transformation of isocitrate into a-ketoglutarate. Wolfson and Williams-Ashman and their collaborators29 3 have demonstrated the presence of this enzyme in human serum and shown that its activity is elevated in cases of hepatitis or metastatic liver cancer but not in cases of obstructive jaundice. By using the same technique of measurement, but at a different temperature, we have demonstrated the presence of this enzyme in the cerebrospinal fluid, and we have studied its variation in pathological conditions of the nervous system, especially in tumors.

MATERIAL AND METHODS

The following reagents were used: (1) sodium isocitrate in a concentration of 32.x 1WM; (2) triphosphopyridine nucleotide (TPN) in a concentration of 1 . 2 9 x l V M ; and

(3) manganese chloride in a concentration of 19.x 1 W M .

T h e buffer solution consisted of 0.2M triethanolamine and hydrochloric acid at pH 7.5.

T h e method of measurement was essentially the same as that used by Wolfson and Williams-Ashman and their co-worker& 3 with the following changes: the measurement was carried out on 2 ml. of spinal fluid in a volume of reaction mixture of 4.1 ml. at 37" C. Distilled water was used as a blank.

Standardization. As in the method of Wolfson and Williams-Ashman and their collabo-