ENZYMES: Catalysis, Kinetics and Mechanisms

✍ Scribed by N.S. Punekar

• Publisher: Springer Singapore
• Year: 2018
• Tongue: English
• Leaves: 560
• Series: Eğitim Tanrısı
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers.

The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

✦ Table of Contents

Front Matter ....Pages i-xxiii
Front Matter ....Pages 1-1
Enzymes: Their Place in Biology (N. S. Punekar)....Pages 3-4
Enzymes: Historical Aspects (N. S. Punekar)....Pages 5-13
Exploiting Enzymes: Technology and Applications (N. S. Punekar)....Pages 15-31
On Enzyme Nomenclature and Classification (N. S. Punekar)....Pages 33-41
Hallmarks of an Enzyme Catalyst (N. S. Punekar)....Pages 43-51
Origins of Enzyme Catalytic Power (N. S. Punekar)....Pages 53-70
Which Enzyme Uses What Tricks? (N. S. Punekar)....Pages 71-74
Structure and Catalysis: Conformational Flexibility and Protein Motion (N. S. Punekar)....Pages 75-82
Front Matter ....Pages 83-83
Chemical Kinetics: Fundamentals (N. S. Punekar)....Pages 85-96
Concepts of Equilibrium and Steady State (N. S. Punekar)....Pages 97-106
ES Complex and Pre-steady-state Kinetics (N. S. Punekar)....Pages 107-114
Principles of Enzyme Assays (N. S. Punekar)....Pages 115-129
Good Kinetic Practices (N. S. Punekar)....Pages 131-142
Quantification of Catalysis and Measures of Enzyme Purity (N. S. Punekar)....Pages 143-153
Henri–Michaelis–Menten Equation (N. S. Punekar)....Pages 155-176
More Complex Rate Expressions (N. S. Punekar)....Pages 177-191
Enzyme Kinetic Data: Collection and Analysis (N. S. Punekar)....Pages 193-211
Front Matter ....Pages 213-213
Approaches to Kinetic Mechanism: An Overview (N. S. Punekar)....Pages 215-219
Analysis of Initial Velocity Patterns (N. S. Punekar)....Pages 221-230
Enzyme Inhibition Analyses (N. S. Punekar)....Pages 231-236
Irreversible Inhibitions (N. S. Punekar)....Pages 237-244
Reversible Inhibitions (N. S. Punekar)....Pages 245-257
Alternate Substrate (Product) Interactions (N. S. Punekar)....Pages 259-266
pH Studies with Enzymes (N. S. Punekar)....Pages 267-274
Isotopes in Enzymology (N. S. Punekar)....Pages 275-279
Isotope Exchanges at Equilibrium (N. S. Punekar)....Pages 281-286
Isotope Effects in Enzymology (N. S. Punekar)....Pages 287-299
From Kinetic Data to Mechanism and Back (N. S. Punekar)....Pages 301-310
Front Matter ....Pages 311-311
Chemical Reactivity and Molecular Interactions (N. S. Punekar)....Pages 313-330
Acid–Base Chemistry and Catalysis (N. S. Punekar)....Pages 331-343
Nucleophilic Catalysis and Covalent Reaction Intermediates (N. S. Punekar)....Pages 345-365
Phosphoryl Group Chemistry and Importance of ATP (N. S. Punekar)....Pages 367-383
Enzymatic Oxidation–Reduction Reactions (N. S. Punekar)....Pages 385-402
Carboxylations and Decarboxylations (N. S. Punekar)....Pages 403-420
Electrophilic Catalysis and Amino Acid Transformations (N. S. Punekar)....Pages 421-436
Integrating Kinetic and Chemical Mechanisms: A Synthesis (N. S. Punekar)....Pages 437-457
Front Matter ....Pages 459-459
Regulation of Enzyme Activity (N. S. Punekar)....Pages 461-492
In Vitro Versus In Vivo: Concepts and Consequences (N. S. Punekar)....Pages 493-519
Future of Enzymology: An Appraisal (N. S. Punekar)....Pages 521-551
Closure – Whither Enzymology (N. S. Punekar)....Pages 553-557
Back Matter ....Pages 559-562

✦ Subjects

Life Sciences; Enzymology; Protein-Ligand Interactions; Biomedical Engineering/Biotechnology; Applied Microbiology; Protein Structure