𝔖 Bobbio Scriptorium
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Enzymes and Genes from the lux Operons of Bioluminescent Bacteria

✍ Scribed by Meighen, E A

Book ID
120754069
Publisher
Annual Reviews
Year
1988
Tongue
English
Weight
980 KB
Volume
42
Category
Article
ISSN
0066-4227

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PCR based gene engineering of the Vibrio
PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products
✍ Hill, Philip J. ;Swift, Simon ;Stewart, Gordon S. A. B. πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 988 KB

The polymerase chain reaction (PCR) was applied to clone the luxA and luxB genes from Vibrio harveyi, and the trp poL (promoter operator leader) region and the trpB and trpA genes from Escherichia coli. PCR-derived luxA/B and trpB/A genes were shown to express bacterial luciferase and tryptophan syn