Enzyme yields from cells of brewer's yeast disrupted by treatment in a horizontal disintegrator
✍ Scribed by Ference Marffy; Maria-Regina Kula
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 472 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Maltase, phosphatase, malate dehydrogenase, glucose‐6‐phosphate dehydrogenase, and 6‐phosphogluconate dehydrogenase activities were determined in the supernatant of brewer's yeast cells from samples taken at various intervals during a 90 min treatment in a Dyno‐Mill disintegrator.
In an attempt to find optimum operating conditions, the following parameters were varied: speed of rotation of the agitator, flow rate, yeast concentration in the slurry and bead diameter, and the specific rate of extraction was determined for each such variation. Analysis of the data showed that, superimposed upon extraction, there was a progressive but slow loss of total activities. Enzyme release could be expressed as an approximately first‐order chemical reaction. The results compared well with those of Follows et al. (Biotechnol. Bioeng.), 13, 549 (1971) and showed that the Dyno‐Mill can be operated under proper conditions without significant loss of enzyme activity and with high yields.