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Enzyme reactor for urinary acylcarnitines assay by reversed-phase high-performance liquid chromatography

✍ Scribed by Kojiro Matsumoto; Mamoru Takahashi; Nobuaki Takiyama; Hideo Misaki; Nobutake Matsuo; Sawao Murano; Hidetaka Yuki


Book ID
115827785
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
646 KB
Volume
216
Category
Article
ISSN
0009-8981

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Assay for ADP-Ribosyl Cyclase by Reverse
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Cyclic ADP-ribose (cADPR), a natural metabolite of beta-NAD(+), is a second messenger for Ca(2+) signaling in T cells. As a tool for purification and identification of ADP-ribosyl cyclase(s) in T cells, a sensitive and specific enzymatic assay using 1,N(6)-etheno-NAD(+) as substrate was developed. A