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Enzyme microelectrodes for choline and acetylcholine and their applications

โœ Scribed by Kenji Kano; Kazuo Morikage; Bunji Uno; Yukihiro Esaka; Masashi Goto

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
571 KB
Volume
299
Category
Article
ISSN
0003-2670

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โœฆ Synopsis

Hydrogen peroxide based amperometric microsensors have been developed for choline (Ch) and acetylcholine (Ach) by coimmobilization of acetylcholinesterase (AchE) and choline oxidase (ChO) on platinum disk microelectrodes using glutaraldehyde vapor. The sensor was applied in micro flow injection and micro liquid chromatographic analyses. The chromatographic peak heights were linearly proportional to the amounts of Ach and Ch over the range of 0.05 to lo3 pmol (8 x lo-' to 1.5 x lo-' M, 0.06 ~1) with correlation coefficients > 0.99. Immobilization of ChO was used for the selective determination of Ch with a response time of 5 s. This electrode was used for activity measurements of AchE down to 0.25 U ml-' in a 1 ~1 volume in a batch method at a total volume of 5.0 ml.

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