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Enzyme-mediated site-specific protein modification

✍ Scribed by Tsutomu Tanaka; Takayuki Sakamoto; Kanako Wakamura; Ryosuke Takase; Takuya Matsumoto; Shiori Sawamoto; Akihiko Kondo

Book ID: 113683020
Publisher: Elsevier Science
Year: 2009
Tongue: English
Weight: 114 KB
Volume: 108
Category: Article
ISSN: 1389-1723
DOI: 10.1016/j.jbiosc.2009.08.282

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## Abstract The use of enzymes is a promising approach for site‐specific protein modification on living cells owing to their substrate specificity. Herein we describe a general strategy for the site‐specific modification of cell surface proteins with synthetic molecules by using Sortase, a transpep