Enzyme-linked lectin assay (ELLA): Use of alkaline phosphatase-conjugated Griffonia simplicifolia B4 isolectin for the detection of α-d-galactopyranosyl end groups
✍ Scribed by J.Philip McCoy Jr.; James Varani; Irwin J. Goldstein
- Book ID
- 102984848
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 613 KB
- Volume
- 130
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Alkaline phosphatase has been coupled to Grlfinia simplicifolia I B4 isolectin using a onestep glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound natural and synthetic glycoproteins bearing a-Dgalactopyranosyl end groups. The extent of reactivity of the AP-GS I-B, with the glycoproteins appears to be proportional to the number of terminal galactosyl residues present. Furthermore, this assay, termed ELLA (enzyme-linked lectin assay), is specifically inhibitable by low-molecular-weight sugars containing terminal a-D-galactosyl groups. The ELLA reactions may be assayed rapidly and objectively by the use of commercially available ELISA-plate readers using standard filters.