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Enzyme-linked immunosorbent assay for secalonic acid D

✍ Scribed by Joseph N. Neucere

Book ID
104773032
Publisher
Springer Netherlands
Year
1986
Tongue
English
Weight
357 KB
Volume
93
Category
Article
ISSN
0301-486X

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✦ Synopsis

A competitive enzyme-linked immunosorbent assay was developed for the xanthone dimer secalonic acid D. The immunogen and enzyme marker were prepared by direct reaction of secalonic acid D with bovine serum albumin and horseradish peroxidase, respectively. The resultant conjugates were characterized by UV/VIS spectra and thin layer chromatography. The hapten:protein ratios in the conjugates were estimated by difference UV/VIS spectra and by fluorescent techniques. Immunization procedures were conducted utilizing New Zealand rabbits over a period of 12 weeks. The competitive enzyme-linked immunosorbent assay on microtiter plates showed that secalonic acid D was detectable within a range of 250-25 000 ng/assay.

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✍ B. Nilsson πŸ“‚ Article πŸ“… 1989 πŸ› Elsevier Science 🌐 English βš– 857 KB

Enzyme-linked immunosorbent assays (ELISAs) are often used nowadays for qualitative and quantitative determinations of macromolecules. The technique has been undergoing continuous refinement since it was first described by Engvall and Perlman (Immunochemistry 1971, 8:871474) and has, at the same tim