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Enzyme-linked immunosorbent assay (ELISA) for antibodies to Clostridium difficile toxins in patients with pseudomembranous colitis and antibiotic-associated diarrhoea

✍ Scribed by Bo Aronsson; Marta Granström; Roland Möllby; Carl Erik Nord


Book ID
118899100
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
413 KB
Volume
60
Category
Article
ISSN
0022-1759

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661 bp coding for the carboxyl end of the large sub-unit of EBV ribonucleotide reductase was cloned into the pMal plasmid vector. Purified recombinant protein was tested in IgG and IgA ELISAs. For the IgG assay, 81 out of 100 NPC patients tested positive, whereas for the IgA assay, 60 tested positiv