Enzyme Kinetics: Principles and Methods

✍ Scribed by Hans Bisswanger

• Publisher: Wiley-VCH
• Year: 2002
• Tongue: English
• Leaves: 268
• Category: Library

✦ Synopsis

This is a user-friendly and comprehensive treatise on enzyme kinetics - indispensable for biochemists, biologists, medical scientists, and chemists working with enzymes, from advanced students to experts in academia and industry. Theory and practice are well-balanced, the relation to the biological system is always emphasized. Theoretical aspects are presented in a way which is also comprehensible for the beginner. An extensive methodological part provides the expert with valuable support in planning and performing laboratory experiments. Contains a CD-ROM with EKI-3, the elaborate and easy-to-use version of the enzyme kinetics practical course.

✦ Table of Contents

Cover Page......Page 1
Enzyme Kinetics......Page 2
Title Page......Page 3
ISBN 352730343X......Page 4
Preface to the Third German Edition......Page 6
Preface to the English Edition......Page 7
Contents (with page links)......Page 8
Symbols and Abbreviations......Page 11
Introduction......Page 13
References......Page 15
1.1 Diffusion......Page 17
1.2 Interaction of Ligands and Macromolecules......Page 22
1.3 Macromolecules with Identical Independent Binding Sites......Page 23
1.4 Macromolecules with Non-Identical, Independent Binding Sites......Page 38
1.5 Macromolecules with Identical, Interacting Binding Sites, Cooperativity......Page 40
1.6 Non-Identical, Interacting Binding Sites......Page 60
1.7 References......Page 61
2.1 Reaction Order......Page 63
2.2 Steady-State Kinetics and the Michaelis-Menten Equation......Page 67
2.3 Analysis of Enzyme Kinetic Data......Page 70
2.4 Reversible Enzyme Reactions......Page 87
2.5 Enzyme Inhibition......Page 92
2.6 Multi-Substrate Reactions......Page 120
2.7 Derivation of Rate Equations of Complex Enzyme Mechanisms......Page 132
2.8 Kinetic Treatment of Allosteric Enzymes......Page 141
2.9 Special Enzyme Mechanisms......Page 143
2.10 pH and Temperature Dependence of Enzymes......Page 151
2.11 Isotope Exchange......Page 158
2.12 Application of Statistical Methods in Enzyme Kinetics......Page 165
2.13 References......Page 170
3.1 Methods for the Investigation of Multiple Equilibria......Page 173
3.2 Electrochemical Methods......Page 192
3.3 Calorimetry......Page 198
3.4 Spectroscopic Methods......Page 200
3.5 Measurement of Fast Reactions......Page 234
3.6 References......Page 253
Index (with page links)......Page 259
Back Page......Page 268