Enzyme inactivation with shearing

✍ Scribed by S. E. Charm; B. L. Wong

• Book ID: 101721334
• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 199 KB
• Volume: 12
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260120615

✦ Synopsis

Abstract

Enzymes subjected to shearing in a viscometer are partially inactivated. It is possible with viscometry to calculate the degree of inactivation that occura when an enzyme solution flows through a capillary tube. When shear rate × exposure time is less than 10^4^, there is little or no inactivation.

The masa average shear‐rate × time or shear, for laminar flow in a cylindrical tube is simply 16L/3D. It is surprising that for a single pass through a tube, the masa average shear is independent of flow rate and shear rate.