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Enzyme-immunoassay in the diagnosis of hepatitis with emphasis on the detection of “e” antigen (HBeAg)

✍ Scribed by M. v. d. Waart; A. Snelting; J. Cichy; G. Wolters; A. Schuurs

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
384 KB
Volume
3
Category
Article
ISSN
0146-6615

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✦ Synopsis

A brief survey of the application of enzyme-immunoassay (EIA) for the detection of hepatitis B surface antigen (HBsAg), hepatitis A, and their corresponding antibodies is given. The preliminary results of a similar EIA for detection of hepatitis B-related "e" antigen (HBeAg) and its antibody (anti-HBe) are reported. This EIA is much more sensitive than immunodiffusion: at least 128 times for HBeAg and at least 512 times for anti-HBe. HBsAg and its antibody do not interfere with the test. Only a few sera strongly positive for rheumatoid factor gave rise to false-positive results, as was demonstrated by a confirmatory test.

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