𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enzyme immobilization by the coulomb force

✍ Scribed by Shintaro Furusaki; Nobuhiro Asai

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
412 KB
Volume
25
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A new immobilization technique has been developed. It involves immobilizing enzymes on a porous polytetrafluoroethylene membrane with a nonporous polyurethane coat by the use of an electrostatic force, i.e. the Coulomb force. The immobilized enzyme can be recovered by supplying a reversed electrical potential. The reaction characteristics of the immobilized amyloglucosidase were studied using maltose as a substrate. The Michaelis constant becomes larger than that of the native enzyme, and depends on the electrical potential gradient in the solution.

πŸ“œ SIMILAR VOLUMES

The coulomb force and the Ampère force
The coulomb force and the Ampère force
✍ Parry Moon; Domina Eberle Spencer πŸ“‚ Article πŸ“… 1954 πŸ› Elsevier Science 🌐 English βš– 489 KB

The paper presents a preliminary study of electrodynamics based on an equation for the force between moving but unaccelerated charges. It is proved that the unique equation for force between current elements, consistent with a summation of forces between charges and the Amp&re experiments, is Amp~re

Bacteriolysis by immobilized enzymes
Bacteriolysis by immobilized enzymes
✍ Isao Karube; Toshiro Suganuma; Shuichi Suzuki πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 387 KB
A thromboresistant surface by enzyme imm
A thromboresistant surface by enzyme immobilization
✍ Nguyen, An-Lac ;Wilkes, Garth L. πŸ“‚ Article πŸ“… 1974 πŸ› John Wiley and Sons 🌐 English βš– 731 KB

## Abstract A process is described for binding brinolase, a fibrinolytie enzyme, to a solid support. Evidence is presented to prove the activity and stability of the bound enzyme at physiological conditions of pH and temperature. A scheme is suggested for preparing suitable material onto whose surf

Immobilization of enzymes by multilayer
Immobilization of enzymes by multilayer microcapsules
✍ Rainer Pommersheim; JΓΌrgen Schrezenmeir; Walter Vogt πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 877 KB

## Abstract A method is described to obtain a new type of microcapsules for immobilization of enzymes or living cells. The wall of these capsules consists of several layers of poly(ethyleneimine) and poly(acrylic acid). The idea is that diverging properties of the whole assembly can be better contr