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Enzyme-gas chromatography: Theoretical and experimental aspects

✍ Scribed by S. Pulvin; M. Sharan; D. Thomas

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
319 KB
Volume
124
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method based on the production of gas by enzymes was developed to determine the concentration of amino acids. The enzyme was immobilized by coreticulation on the external surface of a capillary silicone tube. The gas produced diffused through the silicone membrane to the lumen of the tube and was carried by a vector gas to a gas chromatograph. The amount of measured gas has been shown to be a function of the amino acid concentration. A model of the system that gave good agreement between experimental and calculated values was developed.

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