✦ LIBER ✦

Enzyme design by chemical modification of protein scaffolds

✍ Scribed by Cheng-Min Tann; Dongfeng Qi; Mark D Distefano

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 288 KB
Volume: 5
Category: Article
ISSN: 1367-5931
DOI: 10.1016/s1367-5931(01)00268-x

No coin nor oath required. For personal study only.

✦ Synopsis

Covalent modification methods allow an almost unlimited range of functionality to be introduced into proteins. In concert with genetic techniques, chemical strategies have had significant impact in the field of enzyme design. Major recent developments include introducing catalytic activity into inactive proteins, modifying the selectivity and/or reactivity of existing enzymes and designing novel enzyme-based biosensors. New chemical methods promise to further increase the range of functionality that can be incorporated into proteins. These results suggest that semi-synthetic methods will play a key role in the development of future biocatalysts.

📜 SIMILAR VOLUMES

Chemical modification of nuclear protein

Chemical modification of nuclear proteins by erythropoietin

✍ Dr. Jerry L. Spivak; Lorna Peck 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 414 KB 👁 2 views

## Abstract The spleen of the exhypoxic polycythemic mouse was employed as a model system to study the effect of erythropoietin on enzymes that chemically modify nuclear proteins. At selected time intervals after in vivo administration of erythropoietin, acetyltransferase and methyltransferase acti

Chemical modification of crayfish axons

Chemical modification of crayfish axons by protein crosslinking aldehydes

✍ Peter G. Shrager; Alfred Strickholm; Robert I. Macey 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 English ⚖ 725 KB

A series of aldehydes of varying protein crosslinking strengths have been tested on intact and internally perfused crayfish axons. Non-crosslinking aldehydes have no effect, or cause a gradual decline in resting potential and overshoot with no widening of the spike. Strong crosslinking compounds, su

Signaling protein inhibitors via the com

Signaling protein inhibitors via the combinatorial modification of peptide scaffolds

✍ David S. Lawrence 📂 Article 📅 2005 🏛 Elsevier Science 🌐 English ⚖ 474 KB

Compounds that selectively interfere with protein -protein interactions are not only invaluable as biological reagents, but may ultimately serve as therapeutically useful drugs for the treatment of a wide variety of disease states. However, unlike active site directed inhibitors that bind to a relat

Chemical methods of protein synthesis an

Chemical methods of protein synthesis and modification

✍ John Humphries; Robin E. Offord; Richard A.G. Smith 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 533 KB

Chemical and recombinant methods have continued to complement one another in the synthesis of protein analogues. Chemical methods remain particularly valuable when non-coded modifications are to be introduced, although it has been accepted since the commercialization of semisynthetic human insulin t

Side Chain Selective Chemical Modificati

Side Chain Selective Chemical Modifications of Proteins

✍ Dan S. Tawfik 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 23 KB 👁 1 views

Redox Proteomics (From Protein Modificat

Redox Proteomics (From Protein Modifications to Cellular Dysfunction and Diseases) || Chemical Modification of Proteins by Reactive Oxygen Species

✍ Dalle-Donne, Isabella; Scaloni, Andrea; Butterfield, D. Allan 📂 Article 📅 2006 🏛 John Wiley & Sons, Inc. 🌐 English ⚖ 309 KB 👁 2 views

methodology And Applications Of Redox Proteomics The Relatively New And Rapidly Changing Field Of Redox Proteomics Has The Potential To Revolutionize How We Diagnose Disease, Assess Risks, Determine Prognoses, And Target Therapeutic Strategies For People With Inflammatory And Aging-as