✦ LIBER ✦

Enzyme Complex Amplification - A Signal Amplification Method for Use in Enzyme Immunoassays

✍ Scribed by M.R. Wilson; S.B. Easterbrooksmith

Book ID: 102560457
Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 468 KB
Volume: 209
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1100

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✦ Synopsis

An amplification system for enzyme immunoassays, in which complexes of streptavidin, biotinylated horseradish peroxidase, antibody are used to detect biotinylated target molecules was developed. In enzyme-linked immunosorbent assay (ELISA) experiments this enzyme complex enhancement (ECA) system gave up to a better than two orders of magnitude increase in sensitivity, compared to a horseradish peroxidase-conjugated streptavidin detection system. The ECA system was shown to be applicable to direct detection of biotinylated antigens, as well as in ELISA employing biotinylated antibodies and in sandwich ELISA. The ECA system was also applicable to immunoblots, giving a sensitivity comparable to that obtained using a radiochemical detection system.

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