Enzyme-based bioartificial polymeric materials: the α-amylase-poly(vinyl alcohol) system

✍ Scribed by Cristallini, Caterina; Lazzeri, Luigi; Cascone, Maria Grazia; Polacco, Giovanni; Lupinacci, Domenico; Barbani, Niccoletta

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 444 KB
• Volume: 44
• Category: Article
• ISSN: 0959-8103
• DOI: 10.1002/(sici)1097-0126(199712)44:4<510::aid-pi884>3.0.co;2-0

✦ Synopsis

The possibility of realizing bioartiÐcial polymeric materials from blends of the enzyme a-amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of Ðlm and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X-ray di †ractometry and morphological analysis. A certain degree of interaction through which the a-amylase molecules inÑuence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary signiÐcantly. In addition, hydrogels prepared by a freezeÈthawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity.