Enzyme association with lipidic Langmuir–Blodgett films: Interests and applications in nanobioscience

✍ Scribed by Agnès P. Girard-Egrot; Stéphanie Godoy; Loïc J. Blum

• Book ID: 104011712
• Publisher: Elsevier Science
• Year: 2005
• Tongue: English
• Weight: 566 KB
• Volume: 116
• Category: Article
• ISSN: 0001-8686
• DOI: 10.1016/j.cis.2005.04.006

✦ Synopsis

This review presents the recent advances in the achievement of organized proteo-lipidic nanostructures based on Langmuir-Blodgett technology and their potential applications in the nanobioscience area. By using the self-assembled properties of amphiphilic biomolecules at the air-water interface, the Langmuir-Blodgett (LB) technique offers the possibility to prepare ultrathin layers suitable for biomolecule immobilization at the molecular level. This review will provide a general overview of the enzyme association with preformed Langmuir-Blodgett films in connection with their potential applications in biosensing device developments, and then introduce the design of a new functionalised biomimetic nanostructure with oriented recognition site. The potential applications of such an organized proteo-lipidic nanostructure for biocatalysis investigations of an immobilised enzyme in a biomimetic situation and for the development of bioelectronic devices are finally discussed.