Enzyme and protein mass transfer coefficient in aqueous two-phase systems—I. Spray extraction columns

✍ Scribed by K.Rostami Jafarabad; S.B. Sawant; J.B. Joshi; S.K. Sikdar

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 960 KB
• Volume: 47
• Category: Article
• ISSN: 0009-2509
• DOI: 10.1016/0009-2509(92)80200-v

✦ Synopsis

Ahstraet

-Fractional dispersed phase hold-up and dispersed side mass transfer coefficients for bovine serum albumin (BSA) and amyloglucosidase were measured in 22, 34, 50, 56, 70 and 95 mm i.d. spray columns using salt-polyethylene glycol (potassium phosphate-PEG and sodium sulphatcPEG) systems. Thee&ct of distributor design and column height were investigated. The eIFect of phase compositions of the aqueous phase system was also studied. Empi&al and scmi-&pirical correlations have been developed for fractional dispersed phase hold-up and dispersed side mass transfer coefficients. more than one theoretical stage is required for achieving the desired level of purity. The% requirements are 'Author to whom correspondence should be addressed.