A Method for Activity Staining after Nat
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Jean Rivoal; Christopher R. Smith; Trevor F. Moraes; David H. Turpin; William C.
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Article
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2002
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Elsevier Science
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English
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We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m