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Enzyme activation by antibodies: A method to determine the binding constant of the activating antibody towards one determinant of E. coli β-d-galactosidase

✍ Scribed by Franco Celada; Alberto J.L. Macario; Everly Conway De Macario


Book ID
115942421
Publisher
Elsevier Science
Year
1973
Weight
569 KB
Volume
10
Category
Article
ISSN
0019-2791

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## Abstract Virgin mouse spleen cells cultured __in vitro__ without antigen or mitogen produce a measurable amount of IgM binding __E. coli__ β‐galactosidase (β‐gal). Lipopolysaccharide (LPS) and LPS plus antigen enhance this response, which cannot be considered truly polyclonal since it does not i